EFFECT OF ELEUHTERINE PALMIFOLIA (L) MERR ON TUMOR CELL PROLIFERATION IN SPRAGUE DAWLEY RATS WITH BREAST CANCER
DOI:
https://doi.org/10.29303/jku.v10i4.545Keywords:
Eleuhterine palmifolia (L) Merr, anti-proliferative, sprague-dawleyAbstract
Background : Immunotherapy is widely used to increase the response rate of chemotherapy drugs. Eleuhterine palmifolia (L) Merr or dayak onion are known to contain phytochemical compounds that have antiproliferative effects, and have been used as anti-cancer empirical therapies. This study aimed to prove the antiproliferative effect of Eleuhterine palmifolia (L) Merr extract in Sprague-Dawley rats with breast cancer.
Methods : This study used 18 Sprague-dawley rats, divided into 3 groups, K, P1 and P2. K was given regular feed, P1 received adriamycin 5 mg/kgBW single dose, P2 received adriamycin 5 mg/kgBW single dose and extract of dayak onion 105 mg/kgBW/day for 3 weeks.
Results : Difference in tumor size before and after treatment was 7.2 ± 2.57 mm (K), 3.23 ± 3.03 mm (P1), 3.14 ± 2.65 mm (P2), with p value is 0,157 (p>0,05). Ki-67 index calculations are 11.1 ± 7.27% (K), 9.64 ± 6.99% (P1), 9.58 ± 3.52% (P2), with p value is 0,704 (p>0,05).
Conclusion : The addition of Eleuhterine palmifolia (L) Merr (dayak onion) extract did not cause a significant difference in tumor cell proliferation in Sprague-Dawley rats with breast cancer.
References
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24. Yuniarti A, Sundhani E, Nurulita NA. The potentiation effect of Bawang Dayak (Sisyrinchium palmifolium L .) extract on T47D cell growth inhibition after 5-fluorouracil treatment The potentiation effect of Bawang Dayak (Sisyrinchium palmifolium L .) extract on T47D cell growth inhibition a. Pharmaciana. 2018;8(2):195-204 https://doi.org/10.12928/pharmaciana.v8i2.9480
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2. Kementerian Kesehatan Republik Indonesia. Deteksi Dini Kanker Leher Rahim dan Kanker Payudara di Indonesia 2007-2014. Di dalam: Kementerian Kesehatan Republik Indonesia (Ed). Buletin Jendela Data dan Informasi Kesehatan. Jakarta. 2015
3. Kementerian Kesehatan Republik Indonesia. Panduan Nasional Penanganan Kanker (Kanker Payudara) Jakarta : Komite Nasional Penanggulangan Kanker; 2015
4. Paulus A, Budijitno S2, Issakh B. Efektivitas Ekstrak Artemisia vulgaris sebagai Suplementasi terhadap Kemoterapi Adenokarsinoma Mammae dalam Meningkatkan IL-12 dan Indeks Apoptosis Sel Kanker (Studi pada Mencit C3H yang Diberi Regimen Kemoterapi Adriamycin-Cyclophosphamide). Jurnal Kedokteran Brawijaya. 2018;30(2):81-6
5. Luangdilok S, Samarnthai N, and Korphaisarn K. Association between Pathological Complete Response and Outcome Following Neoadjuvant Chemotherapy in Locally Advanced Breast Cancer Patients. Journal of Breast Cancer. 2014;17(4):376-85.
6. Mutiah R, Hadya CM, Ma’arif B, Bhagawan WS, Annisa R, Indrawijaya YYA, Huwaida FI, Ramadhani R, Susilowati R, Taufik I. Metabolite Fingerprintiing of Eleutherine palmifolia (L.) Merr. by HPTLC-Densitometry and Its Correlation with Anticancer Activities and In Vitro Toxicity. Indonesian Journal Pharmacy. 2019;30(3):157-66
7. Muti’ah R, Listiyana A, Nafisa BB, Suryadinata A. Kajian Efek Ekstrak Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr) sebagai Antikanker. Journal of Islamic Pharmacy. 2020;5(2): p.14-26
8. Apriliani NR, Sulistiani, Sari NE, Mardiah. Analisa Kualitatif Dan Kuantitatif Komponen Bioaktif Hasil Ekstraksi Bawang Dayak. Jurnal Zarah. 2017; 5(1):1-4
9. Narko N, Permana B, Prasetiawati3 R, Soni D , Khairiyah F. Molecular Docking study of Bulb Of Bawang Dayak (Eleutherine Palmifolia (L) Merr)Compound As Anti Servical Cancer. Jurnal Ilmiah Farmako Bahari. 2017; 8(2):1-14
10. Gayatri PR, Sudjarwo SA, I’tishom R. Potensi Ekstrak Etanol Bawang Dayak (Eleutherine americana Merr.) sebagai Protektor Diameter Tubulus Seminiferus Mencit (Mus musculus) Balb/C yang di Induksi Timbal Asetat. Jurnal Biosains Pascasarjana. 2017;19(3):189–96
11. Mutiah R., Widyawaruyanti A, Sukardiman S. Calotroposid A: A glycosides terpenoids from Calotropis gigantea induces apoptosis of colon cancer WiDr cells through cell cycle arrest G2/M and caspase 8 expression. Asian Pacific Journal of Cancer Prevention. 2018;19(6):1457–64. https://doi.org/10.22034/APJCP.2018.19.6.1457.
12. Mutiah R, Choiroh, F, Annisa R, Listiyana A. Combinational effect of Eleutherine palmifolia (L.) merr extract and doxorubicin chemotherapy on HeLa cervical cancer cells. AIP Conference Proceedings, 2020. https://doi.org/10.1063/1.5115718.
13. Utami P, Puspitanisngtyas DE. The Miracle of Herbs. Jakarta : Agro Media Pustaka. 2013:18-23
14. Kinoshita, Yoshioka M, Emoto Y, Yuri T, Yuki M, Koyama C, Takenouchi A, Hamazaki K, Tsubura A, Yoshizawa K. Dietary Effect Of Mead Acid On Dmba Induced Breast Cancer In Female Sprague Dawley Rats. International Journal Of Functional Nutrition. 2020; 1(7). Doi: 10.3892/Ijfn.2020.7.
15. Kirubha SPA, Anburajan M, Venkataraman B, Akila R, Sharath D, Raj B. Evaluation of Mammary Cancer in 7,12-Dimethylbenz(a) anthracene-Induced Wister Rats by Asymmetrical Temperature with SerumCEA Levels and Histopathology. Journal of Biomedicine and Biotechnology. 2012. Doi:10.1155/2012/786417
16. Mizuno Y, Abe H, Seto H, Sato K, Fuchikami H, Natori T, Takeda N, Inoue Y, Yamada, J. Standardized Assessment of Ki-67 in Breast Cancer Patients Using Virtual Slides and an Automated Analyzer in Comparison to Central/Local Pathological Assessments. Journal of Cancer Therapy. 2014; 5:141-6
17. Asri A, Mayorita P, Kham D. Hubungan Ekspresi Ki-67 Dengan Karakteristik Histopatologik Pada Kanker Payudara Tripel Negatif. MKA. 2015;38(3):165-72
18. Stephan P. The Ki-67 Proliferation Marker Test and Breast Cancer Treatment. This test plays a role in predicting chemotherapy response and prognosis. 2021. Available from : https://www.verywellhealth.com/ki-67-tumor-marker-test-430609. 2021.4.14
19. Nahed A. Soliman1, Shaimaa M. Yussif. Ki-67 as a prognostic marker according to breast cancer molecular subtype. Cancer Biol Med. 2016;13(4):497-504
20. Lombardi A, Lazzeroni R, Bersigotti L, Vitale V, Amanti C. The Proper Ki-67 Cut-Off in Hormone Responsive Breast Cancer: A Monoinstitutional Analysis with Long-Term Follow-Up. Dove Press journal : Breast Cancer - Targets and Therapy. 2021;13:213–7
21. Darmayani P, Susraini A, Moestikaningsih. Indeks Mitosis dan Indeks Proliferasi Ki-67 Lebih Tinggi pada Karsinoma Sel Basal Tipe Agresif Dibandingkan Tipe Non Agresif. Majalah Patologi. 2018; 57:24-9
22. Mahayasa IMW, Tobing MDL, Harsono AB. Hubungan Antara Ekspresi Ki-67 Dan Kaspase-3 Dengan Respons Kemoterapi Neoajuvan Pada Pasien Karsinoma Serviks Stadium Ib2 Dan Iia2 Di Rumah Sakit Dr. Hasan Sadikin, Bandung Indonesian Journal Of Cancer. 2016;10(2):49-54
23. Wu CH, Chen HY, Wang CW, Shieh TM, Huang T C, Lin LC, Wang KL, Hsia SM. Isoliquiritigenin induces apoptosis and autophagy and inhibits endometrial cancer growth in mice. Oncotarget. 2016;7(45):73432–47. https://doi.org/10.18632/oncotarget.12369.
24. Yuniarti A, Sundhani E, Nurulita NA. The potentiation effect of Bawang Dayak (Sisyrinchium palmifolium L .) extract on T47D cell growth inhibition after 5-fluorouracil treatment The potentiation effect of Bawang Dayak (Sisyrinchium palmifolium L .) extract on T47D cell growth inhibition a. Pharmaciana. 2018;8(2):195-204 https://doi.org/10.12928/pharmaciana.v8i2.9480
25. Adnyana IK, Sukrasno, Kusmardiayani S. Anti Tumor and Immunostmulant Activities of Eleutherine Americana Exctract and Isolation of Its Active Components. ITB. Contribution to the Nation Competitiveness. 2013:66-9
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Published
2022-01-22
How to Cite
Ratna Widayati, Yan Wisnu Prajoko, & Udadi Sadhana. (2022). EFFECT OF ELEUHTERINE PALMIFOLIA (L) MERR ON TUMOR CELL PROLIFERATION IN SPRAGUE DAWLEY RATS WITH BREAST CANCER. Jurnal Kedokteran, 10(4), 645–651. https://doi.org/10.29303/jku.v10i4.545
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