PENGARUH ELEUHTERINE PALMIFOLIA (L) MERR TERHADAP PROLIFERASI SEL TUMOR PADA TIKUS SPRAGUE DAWLEY DENGAN KANKER PAYUDARA
DOI:
https://doi.org/10.29303/jku.v10i4.545Kata Kunci:
Eleuhterine palmifolia (L) Merr, antiproliferasi, sprague-dawleyAbstrak
Latar belakang : Imunoterapi banyak digunakan untuk meningkatkan response rate dari obat kemoterapi. Eleuhterine palmifolia (L) Merr atau bawang dayak diketahui mengandung senyawa fitokimia yang mempunyai efek antiproliferasi, dan telah digunakan sebagai terapi empiris anti kanker. Penelitian ini bertujuan untuk membuktikan adanya efek antiproliferasi dari ekstrak Eleuhterine palmifolia (L) Merr pada tikus Sprague-Dawley dengan kanker payudara.
Metode : Penelitian ini menggunakan 18 ekor tikus Sprague-dawley, dibagi menjadi 3 kelompok, kelompok K, P1 dan P2. Kelompok K diberi pakan biasa, kelompok P1 diberikan adriamycin 5 mg/kgBB/hari dosis tunggal, kelompok P2 diberikan adriamycin 5 mg/kgBB/hari dosis tunggal dan ekstrak bawang dayak 105 mg/kgBB/hari selama 3 minggu.
Hasil : Selisih ukuran tumor sebelum dan sesudah perlakuan adalah 7,2±2,57 mm (K), 3,23±3,03 mm (P1), 3,14±2,65 mm (P2), dengan nilai p=0,157 (p>0,05). Hasil penghitungan indeks Ki-67 adalah 11,1±7,27% (K), 9,64±6,99% (P1), 9,58±3,52% (P2), dengan nilai p=0,704 (p>0,05).
Kesimpulan : Penambahan ekstrak bawang dayak (Eleuhterine palmifolia (L) Merr ) tidak menyebabkan perbedaan bermakna dalam hal proliferasi sel tumor pada tikus Sprague-dawley dengan kanker payudara.
Referensi
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10. Gayatri PR, Sudjarwo SA, I’tishom R. Potensi Ekstrak Etanol Bawang Dayak (Eleutherine americana Merr.) sebagai Protektor Diameter Tubulus Seminiferus Mencit (Mus musculus) Balb/C yang di Induksi Timbal Asetat. Jurnal Biosains Pascasarjana. 2017;19(3):189–96
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12. Mutiah R, Choiroh, F, Annisa R, Listiyana A. Combinational effect of Eleutherine palmifolia (L.) merr extract and doxorubicin chemotherapy on HeLa cervical cancer cells. AIP Conference Proceedings, 2020. https://doi.org/10.1063/1.5115718.
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14. Kinoshita, Yoshioka M, Emoto Y, Yuri T, Yuki M, Koyama C, Takenouchi A, Hamazaki K, Tsubura A, Yoshizawa K. Dietary Effect Of Mead Acid On Dmba Induced Breast Cancer In Female Sprague Dawley Rats. International Journal Of Functional Nutrition. 2020; 1(7). Doi: 10.3892/Ijfn.2020.7.
15. Kirubha SPA, Anburajan M, Venkataraman B, Akila R, Sharath D, Raj B. Evaluation of Mammary Cancer in 7,12-Dimethylbenz(a) anthracene-Induced Wister Rats by Asymmetrical Temperature with SerumCEA Levels and Histopathology. Journal of Biomedicine and Biotechnology. 2012. Doi:10.1155/2012/786417
16. Mizuno Y, Abe H, Seto H, Sato K, Fuchikami H, Natori T, Takeda N, Inoue Y, Yamada, J. Standardized Assessment of Ki-67 in Breast Cancer Patients Using Virtual Slides and an Automated Analyzer in Comparison to Central/Local Pathological Assessments. Journal of Cancer Therapy. 2014; 5:141-6
17. Asri A, Mayorita P, Kham D. Hubungan Ekspresi Ki-67 Dengan Karakteristik Histopatologik Pada Kanker Payudara Tripel Negatif. MKA. 2015;38(3):165-72
18. Stephan P. The Ki-67 Proliferation Marker Test and Breast Cancer Treatment. This test plays a role in predicting chemotherapy response and prognosis. 2021. Available from : https://www.verywellhealth.com/ki-67-tumor-marker-test-430609. 2021.4.14
19. Nahed A. Soliman1, Shaimaa M. Yussif. Ki-67 as a prognostic marker according to breast cancer molecular subtype. Cancer Biol Med. 2016;13(4):497-504
20. Lombardi A, Lazzeroni R, Bersigotti L, Vitale V, Amanti C. The Proper Ki-67 Cut-Off in Hormone Responsive Breast Cancer: A Monoinstitutional Analysis with Long-Term Follow-Up. Dove Press journal : Breast Cancer - Targets and Therapy. 2021;13:213–7
21. Darmayani P, Susraini A, Moestikaningsih. Indeks Mitosis dan Indeks Proliferasi Ki-67 Lebih Tinggi pada Karsinoma Sel Basal Tipe Agresif Dibandingkan Tipe Non Agresif. Majalah Patologi. 2018; 57:24-9
22. Mahayasa IMW, Tobing MDL, Harsono AB. Hubungan Antara Ekspresi Ki-67 Dan Kaspase-3 Dengan Respons Kemoterapi Neoajuvan Pada Pasien Karsinoma Serviks Stadium Ib2 Dan Iia2 Di Rumah Sakit Dr. Hasan Sadikin, Bandung Indonesian Journal Of Cancer. 2016;10(2):49-54
23. Wu CH, Chen HY, Wang CW, Shieh TM, Huang T C, Lin LC, Wang KL, Hsia SM. Isoliquiritigenin induces apoptosis and autophagy and inhibits endometrial cancer growth in mice. Oncotarget. 2016;7(45):73432–47. https://doi.org/10.18632/oncotarget.12369.
24. Yuniarti A, Sundhani E, Nurulita NA. The potentiation effect of Bawang Dayak (Sisyrinchium palmifolium L .) extract on T47D cell growth inhibition after 5-fluorouracil treatment The potentiation effect of Bawang Dayak (Sisyrinchium palmifolium L .) extract on T47D cell growth inhibition a. Pharmaciana. 2018;8(2):195-204 https://doi.org/10.12928/pharmaciana.v8i2.9480
25. Adnyana IK, Sukrasno, Kusmardiayani S. Anti Tumor and Immunostmulant Activities of Eleutherine Americana Exctract and Isolation of Its Active Components. ITB. Contribution to the Nation Competitiveness. 2013:66-9
2. Kementerian Kesehatan Republik Indonesia. Deteksi Dini Kanker Leher Rahim dan Kanker Payudara di Indonesia 2007-2014. Di dalam: Kementerian Kesehatan Republik Indonesia (Ed). Buletin Jendela Data dan Informasi Kesehatan. Jakarta. 2015
3. Kementerian Kesehatan Republik Indonesia. Panduan Nasional Penanganan Kanker (Kanker Payudara) Jakarta : Komite Nasional Penanggulangan Kanker; 2015
4. Paulus A, Budijitno S2, Issakh B. Efektivitas Ekstrak Artemisia vulgaris sebagai Suplementasi terhadap Kemoterapi Adenokarsinoma Mammae dalam Meningkatkan IL-12 dan Indeks Apoptosis Sel Kanker (Studi pada Mencit C3H yang Diberi Regimen Kemoterapi Adriamycin-Cyclophosphamide). Jurnal Kedokteran Brawijaya. 2018;30(2):81-6
5. Luangdilok S, Samarnthai N, and Korphaisarn K. Association between Pathological Complete Response and Outcome Following Neoadjuvant Chemotherapy in Locally Advanced Breast Cancer Patients. Journal of Breast Cancer. 2014;17(4):376-85.
6. Mutiah R, Hadya CM, Ma’arif B, Bhagawan WS, Annisa R, Indrawijaya YYA, Huwaida FI, Ramadhani R, Susilowati R, Taufik I. Metabolite Fingerprintiing of Eleutherine palmifolia (L.) Merr. by HPTLC-Densitometry and Its Correlation with Anticancer Activities and In Vitro Toxicity. Indonesian Journal Pharmacy. 2019;30(3):157-66
7. Muti’ah R, Listiyana A, Nafisa BB, Suryadinata A. Kajian Efek Ekstrak Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr) sebagai Antikanker. Journal of Islamic Pharmacy. 2020;5(2): p.14-26
8. Apriliani NR, Sulistiani, Sari NE, Mardiah. Analisa Kualitatif Dan Kuantitatif Komponen Bioaktif Hasil Ekstraksi Bawang Dayak. Jurnal Zarah. 2017; 5(1):1-4
9. Narko N, Permana B, Prasetiawati3 R, Soni D , Khairiyah F. Molecular Docking study of Bulb Of Bawang Dayak (Eleutherine Palmifolia (L) Merr)Compound As Anti Servical Cancer. Jurnal Ilmiah Farmako Bahari. 2017; 8(2):1-14
10. Gayatri PR, Sudjarwo SA, I’tishom R. Potensi Ekstrak Etanol Bawang Dayak (Eleutherine americana Merr.) sebagai Protektor Diameter Tubulus Seminiferus Mencit (Mus musculus) Balb/C yang di Induksi Timbal Asetat. Jurnal Biosains Pascasarjana. 2017;19(3):189–96
11. Mutiah R., Widyawaruyanti A, Sukardiman S. Calotroposid A: A glycosides terpenoids from Calotropis gigantea induces apoptosis of colon cancer WiDr cells through cell cycle arrest G2/M and caspase 8 expression. Asian Pacific Journal of Cancer Prevention. 2018;19(6):1457–64. https://doi.org/10.22034/APJCP.2018.19.6.1457.
12. Mutiah R, Choiroh, F, Annisa R, Listiyana A. Combinational effect of Eleutherine palmifolia (L.) merr extract and doxorubicin chemotherapy on HeLa cervical cancer cells. AIP Conference Proceedings, 2020. https://doi.org/10.1063/1.5115718.
13. Utami P, Puspitanisngtyas DE. The Miracle of Herbs. Jakarta : Agro Media Pustaka. 2013:18-23
14. Kinoshita, Yoshioka M, Emoto Y, Yuri T, Yuki M, Koyama C, Takenouchi A, Hamazaki K, Tsubura A, Yoshizawa K. Dietary Effect Of Mead Acid On Dmba Induced Breast Cancer In Female Sprague Dawley Rats. International Journal Of Functional Nutrition. 2020; 1(7). Doi: 10.3892/Ijfn.2020.7.
15. Kirubha SPA, Anburajan M, Venkataraman B, Akila R, Sharath D, Raj B. Evaluation of Mammary Cancer in 7,12-Dimethylbenz(a) anthracene-Induced Wister Rats by Asymmetrical Temperature with SerumCEA Levels and Histopathology. Journal of Biomedicine and Biotechnology. 2012. Doi:10.1155/2012/786417
16. Mizuno Y, Abe H, Seto H, Sato K, Fuchikami H, Natori T, Takeda N, Inoue Y, Yamada, J. Standardized Assessment of Ki-67 in Breast Cancer Patients Using Virtual Slides and an Automated Analyzer in Comparison to Central/Local Pathological Assessments. Journal of Cancer Therapy. 2014; 5:141-6
17. Asri A, Mayorita P, Kham D. Hubungan Ekspresi Ki-67 Dengan Karakteristik Histopatologik Pada Kanker Payudara Tripel Negatif. MKA. 2015;38(3):165-72
18. Stephan P. The Ki-67 Proliferation Marker Test and Breast Cancer Treatment. This test plays a role in predicting chemotherapy response and prognosis. 2021. Available from : https://www.verywellhealth.com/ki-67-tumor-marker-test-430609. 2021.4.14
19. Nahed A. Soliman1, Shaimaa M. Yussif. Ki-67 as a prognostic marker according to breast cancer molecular subtype. Cancer Biol Med. 2016;13(4):497-504
20. Lombardi A, Lazzeroni R, Bersigotti L, Vitale V, Amanti C. The Proper Ki-67 Cut-Off in Hormone Responsive Breast Cancer: A Monoinstitutional Analysis with Long-Term Follow-Up. Dove Press journal : Breast Cancer - Targets and Therapy. 2021;13:213–7
21. Darmayani P, Susraini A, Moestikaningsih. Indeks Mitosis dan Indeks Proliferasi Ki-67 Lebih Tinggi pada Karsinoma Sel Basal Tipe Agresif Dibandingkan Tipe Non Agresif. Majalah Patologi. 2018; 57:24-9
22. Mahayasa IMW, Tobing MDL, Harsono AB. Hubungan Antara Ekspresi Ki-67 Dan Kaspase-3 Dengan Respons Kemoterapi Neoajuvan Pada Pasien Karsinoma Serviks Stadium Ib2 Dan Iia2 Di Rumah Sakit Dr. Hasan Sadikin, Bandung Indonesian Journal Of Cancer. 2016;10(2):49-54
23. Wu CH, Chen HY, Wang CW, Shieh TM, Huang T C, Lin LC, Wang KL, Hsia SM. Isoliquiritigenin induces apoptosis and autophagy and inhibits endometrial cancer growth in mice. Oncotarget. 2016;7(45):73432–47. https://doi.org/10.18632/oncotarget.12369.
24. Yuniarti A, Sundhani E, Nurulita NA. The potentiation effect of Bawang Dayak (Sisyrinchium palmifolium L .) extract on T47D cell growth inhibition after 5-fluorouracil treatment The potentiation effect of Bawang Dayak (Sisyrinchium palmifolium L .) extract on T47D cell growth inhibition a. Pharmaciana. 2018;8(2):195-204 https://doi.org/10.12928/pharmaciana.v8i2.9480
25. Adnyana IK, Sukrasno, Kusmardiayani S. Anti Tumor and Immunostmulant Activities of Eleutherine Americana Exctract and Isolation of Its Active Components. ITB. Contribution to the Nation Competitiveness. 2013:66-9
Unduhan
Diterbitkan
2022-01-22
Cara Mengutip
Ratna Widayati, Yan Wisnu Prajoko, & Udadi Sadhana. (2022). PENGARUH ELEUHTERINE PALMIFOLIA (L) MERR TERHADAP PROLIFERASI SEL TUMOR PADA TIKUS SPRAGUE DAWLEY DENGAN KANKER PAYUDARA. Baphomet University : Situs Slot Online Gacor Terbaik Hari Ini Server Thailand Gampang Maxwin 2024, 10(4), 645–651. https://doi.org/10.29303/jku.v10i4.545
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