Test of Anti-Colonization Activity of Lactic Acid Bacteria from Goat Milk Kefir Against Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria in the Intestines of BALB/c House Mouse
DOI:
https://doi.org/10.29303/jku.v10i4.634Keywords:
Anti-colonization, ESBL-producing E.coli (ESBL-Ec), Lactic acid bacteria.Abstract
Escherichia coli (E.coli) is a type of Gram negative bacteria species which is a normal flora in the digestive tract. However, it can potentially become pathogenic if the number in the digestive tract increases. E.coli infection can cause various inflammatory bowel diseases, such as IBD, Crohn's and ulcerative colitis (Khan et al., 2018). Several studies have found that probiotics can act as preventive and therapeutic agents to eradicate pathogenic bacteria through inhibition of adhesion and colonization. This study is conducted to determine the potential of BALB in inhibiting the colonization of ESBL-producing E.coli in vivo in BALB/c house mouse. The results of the in vivo colonization test show that BALB has good colonization inhibitory activity against ESBL-producing E.coli which is indicated by a decrease in the number of ESBL-producing E.coli colonies in pellet-induced BALB/c house mouse and CFS BAL.
References
1. Khan S, Imran A, Malik A, Chaudhary AA, Rub A, Jan AT, et al. Bacterial imbalance and gut pathologies: Association and contribution of E. coli in inflammatory bowel disease. Crit Rev Clin Lab Sci [Internet]. 2018;56(1):1–17. Available from: https://doi.org/10.1080/10408363.2018.1517144
2. Normaliska R, Sudarwanto MB, Latif H. Pola Resistensi Antibiotik pada Escherichia coli Penghasil ESBL dari Sampel Lingkungan di RPH-R Kota Bogor. Acta Vet Indones. 2019;7(2):42–8.
3. Lubis PN, Ferasyi TR, Rastina, Isa M, Nazaruddin, Etriwati. Angka Prevalensi Cemaran Bakteri Escherichia Coli Pada Tangan Pedagang Daging Ayam Broiler Di Dua Pasar Tradisional Kota Banda Aceh. J Ilm Mhs Vet. 2020;4(3):65–72.
4. Niasono A, Latif H, Purnawarman T. Resistensi Antibiotik Terhadap Bakteri Escherichia Coli yang Diisolasi dari Peternakan Ayam Pedaging di Kabupaten Subang, Jawa Barat. Veteriner [Internet]. 2019;20(2):187–95. Available from: http://ojs.unud.ac.id/index.php/jvet
5. World Health Organization. Antimicrobial Resistance. 2020;
6. Martin RM, Bachman MA. Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Front Cell Infect Microbiol. 2018;8:1–15.
7. Pamer EG. Resurrecting the intestinal microbiota to combat antibiotic- resistant pathogens. Science (80- ). 2016;352(6285):535–8.
8. John SAE, Wardoyo EH, Triani E. Hubungan Durasi Kontak Harian Dan Lama Kerja Terhadap Kolonisasi E.coli PenghasilExtended Spectrum Beta Lactamase (ESBL-Ec) Pada Peternak Ayam Di Desa Unggas Teruwai, Kabupaten Lombok Tengah. Program Studi Pendidikan Dokter Fakultas Kedokteran Universitas Mataram; 2020.
9. Maldonado NC, Silva De Ruiz C, Cecilia M, Nader-Macias ME. A simple technique to detect Klebsiella biofilm-forming-strains. Inhibitory potential of Lactobacillus fermentum CRL 1058 whole cells and products. Commun Curr Res Educ Top Trends Appl Microbiol. 2007;(May 2014):52–9.
10. Lagrafeuille R, Miquel S, Balestrino D, Vareille-Delarbre M, Chain F, Langella P, et al. Opposing effect of Lactobacillus on in vitro Klebsiella pneumoniae in biofilm and in an in vivo intestinal colonisation model. Benef Microbes. 2017;9(1):87–100.
11. Lai YC, Lin AC, Chiang MK, Dai YH, Hsu CC, Lu MC, et al. Genotoxic Klebsiella pneumoniae in Taiwan. PLoS One. 2014;9(5):1–9.
12. Jean SS, Lee NY, Tang HJ, Lu MC, Ko WC, Hsueh PR. Carbapenem-resistant enterobacteriaceae infections: Taiwan aspects. Front Microbiol. 2018;9(NOV):1–11.
13. Rodriguez-Baño J, Gutiérrez-Gutiérrez B, Machuca I, Pascual A. Treatment of Infections Caused by Extended-Spectrum-Beta-. Clin Microbiol Rev. 2018;31(2):1–42.
14. Tang H-L, Lai Y-C, Chiou C-S, Liu P-Y, Weng L-L, Hou W, et al. Liver abscess caused by Klebsiella pneumoniae in a red-footed tortoise. J Microbiol Immunol Infect. 2015;1–3.
15. Brooks GF, Carroll KC, Butel JS, Morse SA. Jawetz, Melnick & Adelberg’s Medical Microbiology. 24th editi. McGraw-Hil; 2007. 273–275 p.
16. Georgieva R, Yocheva L, Tserovska L, Zhelezova G, Stefanova N, Atanasova A, et al. Antimicrobial activity and antibiotic susceptibility of Lactobacillus and Bifidobacterium spp. intended for use as starter and probiotic cultures. Biotechnol Biotechnol Equip. 2015;29(1):84–91.
17. Raras TYM, Firdausy AF, Kinanti IR, Noorhamdani N. Anti-Biofilm Activity of Lactic Acid Bacteria Isolated from Kefir against Multidrug-Resistant Klebsiella pneumoniae. J Pure Appl Microbiol. 2019;13(2):983–92.
18. Russell NJ, Gould GW, editors. Food Preservatives. New York: Springer Science & Business Media; 2012. 294 p.
19. Improving Food Safety Through a One Health Approach: Workshop Summary. Washington (DC): National Academies Press (US); 2012.
20. Kasra-Kermanshahi R, Mobarak-Qamsari E. Inhibition effect of Lactic acid bacteria against food born pathogen, Listeria monocytogenes. Appl Food Biotechnol. 2015;2(4):11–9.
21. Bosch M, Rodriguez M, Garcia F, Fernández E, Fuentes MC, Cuñé J. Probiotic properties of Lactobacillus plantarum CECT 7315 and CECT 7316 isolated from faeces of healthy children. Lett Appl Microbiol. 2012;54(3):240–6.
22. Rodrigues L, Banat IM, Teixeira J, Oliveira R. Biosurfactants: Potential applications in medicine. J Antimicrob Chemother. 2006;57(4):609–18.
23. Shokouhfard M, Kermanshahi RK, Shahandashti RV, Feizabadi M, Teimourian S. The inhibitory effect of a Lactobacillus acidophilus derived biosurfactant on biofilm producer Serratia marcescens. Iran J Basic Med Sci. 2015;18(19):1001–7.
24. McFarland L V. Probiotics for the primary and secondary prevention of C. difficile infections: A meta-analysis and systematic review. Antibiotics. 2015;4(2):160–78.
25. Mirnejad R, Vahdati AR, Rashidiani J, Erfani M, Piranfar V. The antimicrobial effect of lactobacillus casei culture supernatant against multiple drug resistant clinical isolates of Shigella sonnei and Shigella flexneri in vitro. Iran Red Crescent Med J. 2013;15(2):122–6.
26. Ahn KB, Baik JE, Park O-J, Yun C-H, Han SH. Lactobacillus plantarum lipoteichoic acid inhibits biofilm formation of Streptococcus mutans. PLoS One. 2018;13(2).
27. Jamalifar H, Rahimi HR, Samadi N, Shaverdi AR, Sharifian Z, Hosseini F, et al. Antimicrobial activity of different Lactobacillus species against multi-drug resistant clinical isolates of Pseudomonas aeruginosa. Iran J Microbiol. 2011;3(1):21–5.
28. Deriu E, Liu JZ, Pezeshki M, Edwards RA, Ochoa RJ, Contreras H, et al. Probiotic bacteria reduce salmonella typhimurium intestinal colonization by competing for iron. Cell Host Microbe. 2013;14(1):26–37.
2. Normaliska R, Sudarwanto MB, Latif H. Pola Resistensi Antibiotik pada Escherichia coli Penghasil ESBL dari Sampel Lingkungan di RPH-R Kota Bogor. Acta Vet Indones. 2019;7(2):42–8.
3. Lubis PN, Ferasyi TR, Rastina, Isa M, Nazaruddin, Etriwati. Angka Prevalensi Cemaran Bakteri Escherichia Coli Pada Tangan Pedagang Daging Ayam Broiler Di Dua Pasar Tradisional Kota Banda Aceh. J Ilm Mhs Vet. 2020;4(3):65–72.
4. Niasono A, Latif H, Purnawarman T. Resistensi Antibiotik Terhadap Bakteri Escherichia Coli yang Diisolasi dari Peternakan Ayam Pedaging di Kabupaten Subang, Jawa Barat. Veteriner [Internet]. 2019;20(2):187–95. Available from: http://ojs.unud.ac.id/index.php/jvet
5. World Health Organization. Antimicrobial Resistance. 2020;
6. Martin RM, Bachman MA. Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Front Cell Infect Microbiol. 2018;8:1–15.
7. Pamer EG. Resurrecting the intestinal microbiota to combat antibiotic- resistant pathogens. Science (80- ). 2016;352(6285):535–8.
8. John SAE, Wardoyo EH, Triani E. Hubungan Durasi Kontak Harian Dan Lama Kerja Terhadap Kolonisasi E.coli PenghasilExtended Spectrum Beta Lactamase (ESBL-Ec) Pada Peternak Ayam Di Desa Unggas Teruwai, Kabupaten Lombok Tengah. Program Studi Pendidikan Dokter Fakultas Kedokteran Universitas Mataram; 2020.
9. Maldonado NC, Silva De Ruiz C, Cecilia M, Nader-Macias ME. A simple technique to detect Klebsiella biofilm-forming-strains. Inhibitory potential of Lactobacillus fermentum CRL 1058 whole cells and products. Commun Curr Res Educ Top Trends Appl Microbiol. 2007;(May 2014):52–9.
10. Lagrafeuille R, Miquel S, Balestrino D, Vareille-Delarbre M, Chain F, Langella P, et al. Opposing effect of Lactobacillus on in vitro Klebsiella pneumoniae in biofilm and in an in vivo intestinal colonisation model. Benef Microbes. 2017;9(1):87–100.
11. Lai YC, Lin AC, Chiang MK, Dai YH, Hsu CC, Lu MC, et al. Genotoxic Klebsiella pneumoniae in Taiwan. PLoS One. 2014;9(5):1–9.
12. Jean SS, Lee NY, Tang HJ, Lu MC, Ko WC, Hsueh PR. Carbapenem-resistant enterobacteriaceae infections: Taiwan aspects. Front Microbiol. 2018;9(NOV):1–11.
13. Rodriguez-Baño J, Gutiérrez-Gutiérrez B, Machuca I, Pascual A. Treatment of Infections Caused by Extended-Spectrum-Beta-. Clin Microbiol Rev. 2018;31(2):1–42.
14. Tang H-L, Lai Y-C, Chiou C-S, Liu P-Y, Weng L-L, Hou W, et al. Liver abscess caused by Klebsiella pneumoniae in a red-footed tortoise. J Microbiol Immunol Infect. 2015;1–3.
15. Brooks GF, Carroll KC, Butel JS, Morse SA. Jawetz, Melnick & Adelberg’s Medical Microbiology. 24th editi. McGraw-Hil; 2007. 273–275 p.
16. Georgieva R, Yocheva L, Tserovska L, Zhelezova G, Stefanova N, Atanasova A, et al. Antimicrobial activity and antibiotic susceptibility of Lactobacillus and Bifidobacterium spp. intended for use as starter and probiotic cultures. Biotechnol Biotechnol Equip. 2015;29(1):84–91.
17. Raras TYM, Firdausy AF, Kinanti IR, Noorhamdani N. Anti-Biofilm Activity of Lactic Acid Bacteria Isolated from Kefir against Multidrug-Resistant Klebsiella pneumoniae. J Pure Appl Microbiol. 2019;13(2):983–92.
18. Russell NJ, Gould GW, editors. Food Preservatives. New York: Springer Science & Business Media; 2012. 294 p.
19. Improving Food Safety Through a One Health Approach: Workshop Summary. Washington (DC): National Academies Press (US); 2012.
20. Kasra-Kermanshahi R, Mobarak-Qamsari E. Inhibition effect of Lactic acid bacteria against food born pathogen, Listeria monocytogenes. Appl Food Biotechnol. 2015;2(4):11–9.
21. Bosch M, Rodriguez M, Garcia F, Fernández E, Fuentes MC, Cuñé J. Probiotic properties of Lactobacillus plantarum CECT 7315 and CECT 7316 isolated from faeces of healthy children. Lett Appl Microbiol. 2012;54(3):240–6.
22. Rodrigues L, Banat IM, Teixeira J, Oliveira R. Biosurfactants: Potential applications in medicine. J Antimicrob Chemother. 2006;57(4):609–18.
23. Shokouhfard M, Kermanshahi RK, Shahandashti RV, Feizabadi M, Teimourian S. The inhibitory effect of a Lactobacillus acidophilus derived biosurfactant on biofilm producer Serratia marcescens. Iran J Basic Med Sci. 2015;18(19):1001–7.
24. McFarland L V. Probiotics for the primary and secondary prevention of C. difficile infections: A meta-analysis and systematic review. Antibiotics. 2015;4(2):160–78.
25. Mirnejad R, Vahdati AR, Rashidiani J, Erfani M, Piranfar V. The antimicrobial effect of lactobacillus casei culture supernatant against multiple drug resistant clinical isolates of Shigella sonnei and Shigella flexneri in vitro. Iran Red Crescent Med J. 2013;15(2):122–6.
26. Ahn KB, Baik JE, Park O-J, Yun C-H, Han SH. Lactobacillus plantarum lipoteichoic acid inhibits biofilm formation of Streptococcus mutans. PLoS One. 2018;13(2).
27. Jamalifar H, Rahimi HR, Samadi N, Shaverdi AR, Sharifian Z, Hosseini F, et al. Antimicrobial activity of different Lactobacillus species against multi-drug resistant clinical isolates of Pseudomonas aeruginosa. Iran J Microbiol. 2011;3(1):21–5.
28. Deriu E, Liu JZ, Pezeshki M, Edwards RA, Ochoa RJ, Contreras H, et al. Probiotic bacteria reduce salmonella typhimurium intestinal colonization by competing for iron. Cell Host Microbe. 2013;14(1):26–37.
Downloads
Published
2022-01-22
How to Cite
Qurrata’yuni Pratiwi, Eustachius Hagni Wardoyo, & Eva Triani. (2022). Test of Anti-Colonization Activity of Lactic Acid Bacteria from Goat Milk Kefir Against Extended Spectrum Beta Lactamase-Producing Escherichia coli Bacteria in the Intestines of BALB/c House Mouse. Jurnal Kedokteran, 10(4), 613–619. https://doi.org/10.29303/jku.v10i4.634
Issue
Section
Research
