UJI AKTIVITAS ANTI KOLONISASI BAKTERI ASAM LAKTAT DARI KEFIR SUSU KAMBING TERHADAP BAKTERI Escherichia coli PENGHASIL Extended Spectrum Beta Lactamase PADA USUS MENCIT BALB/c
DOI:
https://doi.org/10.29303/jku.v10i4.634Kata Kunci:
Anti kolonisasi, Escherichia coli penghasil ESBL (ESBL-Ec), Bakteri asam laktatAbstrak
Escherichia coli (E.coli) adalah salah satu jenis spesies bakteri Gram negatif yang merupakan flora normal pada saluran pencernaan. Namun, berpotensi dapat menjadi patogen apabila jumlahnya dalam saluran pencernaan meningkat. Terinfeksi E.coli dapat menimbulkan beragam penyakit radang usus seperti IBD, Crohn dan kolitis ulserativA. Beberapa studi menemukan bahwa probiotik dapat berperan sebagai agen pencegahan dan terapeutik untuk mengeradikasi bakteri patogen melalui penghambatan adhesi dan kolonisasi. Penelitian ini dilakukan bertujuan untuk mengetahui potensi BAL dalam menghambat kolonisasi E.coli penghasil ESBL secara in-vivo pada mencit BALB/c. Hasil penelitian uji kolonisasi secara in vivo menunjukkan bahwa BAL memiliki aktivitas penghambatan kolonisasi yang baik terhadap E.coli penghasil ESBL yang ditunjukkan melalui penurunan jumlah koloni E.coli penghasil ESBL pada mencit BALB/c yang di induksi pelet dan CFS BAL.
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2. Normaliska R, Sudarwanto MB, Latif H. Pola Resistensi Antibiotik pada Escherichia coli Penghasil ESBL dari Sampel Lingkungan di RPH-R Kota Bogor. Acta Vet Indones. 2019;7(2):42–8.
3. Lubis PN, Ferasyi TR, Rastina, Isa M, Nazaruddin, Etriwati. Angka Prevalensi Cemaran Bakteri Escherichia Coli Pada Tangan Pedagang Daging Ayam Broiler Di Dua Pasar Tradisional Kota Banda Aceh. J Ilm Mhs Vet. 2020;4(3):65–72.
4. Niasono A, Latif H, Purnawarman T. Resistensi Antibiotik Terhadap Bakteri Escherichia Coli yang Diisolasi dari Peternakan Ayam Pedaging di Kabupaten Subang, Jawa Barat. Veteriner [Internet]. 2019;20(2):187–95. Available from: http://ojs.unud.ac.id/index.php/jvet
5. World Health Organization. Antimicrobial Resistance. 2020;
6. Martin RM, Bachman MA. Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Front Cell Infect Microbiol. 2018;8:1–15.
7. Pamer EG. Resurrecting the intestinal microbiota to combat antibiotic- resistant pathogens. Science (80- ). 2016;352(6285):535–8.
8. John SAE, Wardoyo EH, Triani E. Hubungan Durasi Kontak Harian Dan Lama Kerja Terhadap Kolonisasi E.coli PenghasilExtended Spectrum Beta Lactamase (ESBL-Ec) Pada Peternak Ayam Di Desa Unggas Teruwai, Kabupaten Lombok Tengah. Program Studi Pendidikan Dokter Fakultas Kedokteran Universitas Mataram; 2020.
9. Maldonado NC, Silva De Ruiz C, Cecilia M, Nader-Macias ME. A simple technique to detect Klebsiella biofilm-forming-strains. Inhibitory potential of Lactobacillus fermentum CRL 1058 whole cells and products. Commun Curr Res Educ Top Trends Appl Microbiol. 2007;(May 2014):52–9.
10. Lagrafeuille R, Miquel S, Balestrino D, Vareille-Delarbre M, Chain F, Langella P, et al. Opposing effect of Lactobacillus on in vitro Klebsiella pneumoniae in biofilm and in an in vivo intestinal colonisation model. Benef Microbes. 2017;9(1):87–100.
11. Lai YC, Lin AC, Chiang MK, Dai YH, Hsu CC, Lu MC, et al. Genotoxic Klebsiella pneumoniae in Taiwan. PLoS One. 2014;9(5):1–9.
12. Jean SS, Lee NY, Tang HJ, Lu MC, Ko WC, Hsueh PR. Carbapenem-resistant enterobacteriaceae infections: Taiwan aspects. Front Microbiol. 2018;9(NOV):1–11.
13. Rodriguez-Baño J, Gutiérrez-Gutiérrez B, Machuca I, Pascual A. Treatment of Infections Caused by Extended-Spectrum-Beta-. Clin Microbiol Rev. 2018;31(2):1–42.
14. Tang H-L, Lai Y-C, Chiou C-S, Liu P-Y, Weng L-L, Hou W, et al. Liver abscess caused by Klebsiella pneumoniae in a red-footed tortoise. J Microbiol Immunol Infect. 2015;1–3.
15. Brooks GF, Carroll KC, Butel JS, Morse SA. Jawetz, Melnick & Adelberg’s Medical Microbiology. 24th editi. McGraw-Hil; 2007. 273–275 p.
16. Georgieva R, Yocheva L, Tserovska L, Zhelezova G, Stefanova N, Atanasova A, et al. Antimicrobial activity and antibiotic susceptibility of Lactobacillus and Bifidobacterium spp. intended for use as starter and probiotic cultures. Biotechnol Biotechnol Equip. 2015;29(1):84–91.
17. Raras TYM, Firdausy AF, Kinanti IR, Noorhamdani N. Anti-Biofilm Activity of Lactic Acid Bacteria Isolated from Kefir against Multidrug-Resistant Klebsiella pneumoniae. J Pure Appl Microbiol. 2019;13(2):983–92.
18. Russell NJ, Gould GW, editors. Food Preservatives. New York: Springer Science & Business Media; 2012. 294 p.
19. Improving Food Safety Through a One Health Approach: Workshop Summary. Washington (DC): National Academies Press (US); 2012.
20. Kasra-Kermanshahi R, Mobarak-Qamsari E. Inhibition effect of Lactic acid bacteria against food born pathogen, Listeria monocytogenes. Appl Food Biotechnol. 2015;2(4):11–9.
21. Bosch M, Rodriguez M, Garcia F, Fernández E, Fuentes MC, Cuñé J. Probiotic properties of Lactobacillus plantarum CECT 7315 and CECT 7316 isolated from faeces of healthy children. Lett Appl Microbiol. 2012;54(3):240–6.
22. Rodrigues L, Banat IM, Teixeira J, Oliveira R. Biosurfactants: Potential applications in medicine. J Antimicrob Chemother. 2006;57(4):609–18.
23. Shokouhfard M, Kermanshahi RK, Shahandashti RV, Feizabadi M, Teimourian S. The inhibitory effect of a Lactobacillus acidophilus derived biosurfactant on biofilm producer Serratia marcescens. Iran J Basic Med Sci. 2015;18(19):1001–7.
24. McFarland L V. Probiotics for the primary and secondary prevention of C. difficile infections: A meta-analysis and systematic review. Antibiotics. 2015;4(2):160–78.
25. Mirnejad R, Vahdati AR, Rashidiani J, Erfani M, Piranfar V. The antimicrobial effect of lactobacillus casei culture supernatant against multiple drug resistant clinical isolates of Shigella sonnei and Shigella flexneri in vitro. Iran Red Crescent Med J. 2013;15(2):122–6.
26. Ahn KB, Baik JE, Park O-J, Yun C-H, Han SH. Lactobacillus plantarum lipoteichoic acid inhibits biofilm formation of Streptococcus mutans. PLoS One. 2018;13(2).
27. Jamalifar H, Rahimi HR, Samadi N, Shaverdi AR, Sharifian Z, Hosseini F, et al. Antimicrobial activity of different Lactobacillus species against multi-drug resistant clinical isolates of Pseudomonas aeruginosa. Iran J Microbiol. 2011;3(1):21–5.
28. Deriu E, Liu JZ, Pezeshki M, Edwards RA, Ochoa RJ, Contreras H, et al. Probiotic bacteria reduce salmonella typhimurium intestinal colonization by competing for iron. Cell Host Microbe. 2013;14(1):26–37.
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Diterbitkan
2022-01-22
Cara Mengutip
Qurrata’yuni Pratiwi, Eustachius Hagni Wardoyo, & Eva Triani. (2022). UJI AKTIVITAS ANTI KOLONISASI BAKTERI ASAM LAKTAT DARI KEFIR SUSU KAMBING TERHADAP BAKTERI Escherichia coli PENGHASIL Extended Spectrum Beta Lactamase PADA USUS MENCIT BALB/c. Baphomet University : Situs Slot Online Gacor Terbaik Hari Ini Server Thailand Gampang Maxwin 2024, 10(4), 613–619. https://doi.org/10.29303/jku.v10i4.634
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