Analysis of Protective Effects of Forest Honey (Apis dorsata) on Villi Height and Cryptus Depth of Duodenum in Wistar Male Rat Exposed to Aspirin
DOI:
https://doi.org/10.29303/jku.v12i1.884Keywords:
antioxidant, aspirin, duodenum, forest honeyAbstract
Backgound: Peptic ulcer is the main side effect of aspirin usage, which is a drug from the NSAID class. Honey contains flavonoids which has antioxidant properties that is able to prevent and eliminate oxidative damage. Flavonoids can defend villi from damage of duodenal structure.
Method: This study is conducted as an experimental study with a post test only control group design. The sample is stored biological material in the form of preparations of duodenal histological of rats that have been treated and divided into 5 groups: normal control group (KN), treatment group I (KP1) (aspirin 400mg / kgBB + honey 1.55ml / kgBB), treatment group II (P2) (aspirin 400mg / kgBW + honey 3.1 ml / kgBW), positive control (K +) (sucralfate 4 ml / kgBW), and negative control group (K-) (aspirin 400mg / kgBW). The variables in this study were villi height, crypt depth, and villi: duodenal crypt ratio, observed with 10x magnification of the objective lens. Data were analyzed using ANOVA test and LSD post-hoc test, as well as Kruskal-Wallis test with Mann-Whitney post- hoc test.
Result: 1) The group of rats that were given honey at doses of 1.55 ml/kg, 3.1 ml/kg, and aspirin had higher villi, better crypt, and had a better ratio than the duodenal of rats that were only given aspirin.
Conclusion: The provision of forest honey (Apis dorsata) gave a protective effect to rats duodenum which was exposed to aspirin.
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28. Longo DL, Fauci, Anthony S. Harrison’s Gastroenterology and Hepatology. Jakarta: EGC; 2014.
29. Prasetyo A. Uji Efek Protektif Madu Pada Jaringan Duodenum Tikus Jantan Galur Wistas Yang diberi Pajanan Aspirin. J Kedokt Unram. 2022;11(2).
30. Mota K, Dias G, Pinto M, Luiz-Ferreira A, Souza-Brito A, Hiruma-Lima C, et al. Flavonoids with gastroprotective activity. Molecules. 2009;14(3):979–1012.
31. Wang T, Li Q, Bi K. Bioactive flavonoids in medicinal plants: Structure, activity and biological fate. Asian J Pharm Sci. 2018;13(1):12–23.
32. Masyhur M, Handono K, Fitri LE, Indra MR. Quercetin sebagai Penghambat Aktivasi NF-?? dan Penurunan Kadar MCP-1 pada Kultur HUVECs yang Dipapar dengan Leptin. J Kedokt Braijaya. 2011;26(4).
33. Akhlaghi M, Bandy B. Mechanisms of flavonoid protection against myocardial ischemia-reperfusion injury. J od Cell Cardiol. 2009;46(3):309–217.
34. Adikwu E, Deo O. Hepatoprotective Effect of Vitamin C (Ascorbic Acid). Pharmacol Pharm. 2013;4(1):84–92.
2. Eroschenko VP, Fiore MSH di. DiFiore’s Atlas of Histology With Functional Correlations. 12th ed. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins; 2013.
3. Gartner LP. Textbook of Histology. 4th ed. Philadelphia: Elsevier; 2017.
4. Setiawan H, Utami LB, Zulfikar M. Serbuk Daun Jambu Biji Memperbaiki Performans Pertumbuhan dan Morfologi Duodenum Ayam Jawa Super. J Vet. 2018;19(4):554-.
5. Rajput N, Naeem M, Yan R, Xiang Z. Effect of Dietary Supplementation of Curcumin on Growth Performance, Intestinal Morphology and Nutrients Utilization of Broiler Chicks. J Poult Sci. 2013;50(1):44–52.
6. Sieo C, Abdullah N, Tan W, Ho Y. Influence of beta-glucanase-producing Lactobacillus strains on intestinal characteristics and feed passage rate of broiler chickens. Poult Sci. 2005;84(5):734–41.
7. Vincent A, Trianto HF, Ilmiawan MI. Pengaruh Pajanan Monosodium Glutamat terhadap Histologi Duodenum Tikus Putih. eJournal Kedokt Indones. 2014;2(3).
8. Flood P, Rathmell J, Shafer S. Stoelting’s Pharmacology & Physiology in Anesthetic Practice. 5th ed. Philadelphia: Wolters Kluwers Health; 2015.
9. Lovell A, Ernst M. Drug-Induced Hypertension: Focus on Mechanisms and Management. Curr Hypertens Rep. 2017;19(5).
10. Diyah NW, Siswandono. Docking Molekul dan Sintesis Turunan Asam Benzoil Salisilat Tersubstitusi Klor Sebagai Penghambat Siklooksigenase-2. Berk Ilm Kim Farm. 2014;3.
11. Nuraeni D. Pengaruh Pemberian Aspirin Doss Toksik per Oral terhadap Histopatologi Gaster, Duodenum, dan Jejunum Tikus Wistar [Internet]. Universitas Diponegoro; 2007. Available from: http://eprints.undip.ac.id/22600/
12. Katzung BG, Masters SB, Trevor AJ. Basic & clinical pharmacology. New York: McGraw-Hill Medical; 2012.
13. Kuna L, Jakab J, Smolic R, Raguz-Lucic N, Vcev A, Smolic M. Peptic Ulcer Disease: A Brief Review of Conventional Therapy and Herbal Treatment Options. J Clin Med. 2019;8(2):179.
14. Gebermariam T, Brhane G. Determination of quality and adulteration effects of honey from adigrat and its surrounding areas. Int J Technol Enchancements Emerg Res. 2014;2(10).
15. Khalil I, Moniruzzaman M, Boukraa L, Benhanifia M, Islam A, Islam N, et al. hysicochemical and antioxidant properties of Algerian honey. Molecules. 2012;17(9):11199–215.
16. Hardiningtyas S, Purwaningsih S, Handharyani E. Aktivitas Antioksidan dan Efek Hepotoprotektif Daun Bakau Api-api Putih. Pengolah Has Perikan Indones. 2014;17(1).
17. Kumar V, Abbas A, Aster J. Buku Ajar Patologi Robbins. 9th ed. Nasar IM, Cornain S, editors. Singapura: Saunders; 2015.
18. Wiati FF, Armaliana D. Pengaruh Madu Terhadap Gambaran Mikroskopis Duodenum Pada Tikus Wistar Yang Diberi Pajanan Monosodium Glutamat. Universitas Diponegoro; 2015.
19. Nair A, Jacob S. A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm. 2016;7(2):27–31.
20. Al Fady M. Madu dan Luka Diabetik. Yogyakarta: Gosyen Publishing; 2015.
21. Azwar W. Rencana Pengelolaan Hutan Jangka Panjang KPH Model Kapuas Hulu 2014-2024. Mataso: Kesatuan Pengelolaa Hutan Model Kapuas Hulu; 2014.
22. Wulansari D. Madu Sebagai Terapi Komplementer. Yogyakarta: Graha Ilmu; 2018.
23. Nurmila N, Sinay H, Watuguly T. Identifikasi dan Analisis Kadar flavonoid Ekstrak Getah Angsana (Pterocarpus indicus Willd) di Dusun Wanath Kecamatan Leihitu Kabupaten Maluku Tengah. J Biol Pendidik dan Terap. 2019;5(2):65–71.
24. Azizah D nUr, Kumolowati E, Faramayuda F. Penetapan Kadar Flavonoid Metode AlCl3 pada Ekstrak Metanol Kulit Buah Kakao (thebroma cacao L.). Kartika J Ilm Farm. 2014;2(2).
25. Cryer B, Mahaffey K. Gastrointestinal ulcers, role of aspirin, and clinical outcomes: pathobiology, diagnosis, and treatment. J Multidiscip Healthc. 2014;7:137–46.
26. Bintari G, Windarti I, Fiana D. Temulawak (Curcuma xanthorrhiza Roxb) as gastroprotector of mucosal cell damage. Med J Lampung Univ. 2014;3(5):77–84.
27. Shcellack N. An overview of gastropathy induced by nonsteroidal anti-inflammatory drugs. South African Pharm J. 2012;79(4):12–8.
28. Longo DL, Fauci, Anthony S. Harrison’s Gastroenterology and Hepatology. Jakarta: EGC; 2014.
29. Prasetyo A. Uji Efek Protektif Madu Pada Jaringan Duodenum Tikus Jantan Galur Wistas Yang diberi Pajanan Aspirin. J Kedokt Unram. 2022;11(2).
30. Mota K, Dias G, Pinto M, Luiz-Ferreira A, Souza-Brito A, Hiruma-Lima C, et al. Flavonoids with gastroprotective activity. Molecules. 2009;14(3):979–1012.
31. Wang T, Li Q, Bi K. Bioactive flavonoids in medicinal plants: Structure, activity and biological fate. Asian J Pharm Sci. 2018;13(1):12–23.
32. Masyhur M, Handono K, Fitri LE, Indra MR. Quercetin sebagai Penghambat Aktivasi NF-?? dan Penurunan Kadar MCP-1 pada Kultur HUVECs yang Dipapar dengan Leptin. J Kedokt Braijaya. 2011;26(4).
33. Akhlaghi M, Bandy B. Mechanisms of flavonoid protection against myocardial ischemia-reperfusion injury. J od Cell Cardiol. 2009;46(3):309–217.
34. Adikwu E, Deo O. Hepatoprotective Effect of Vitamin C (Ascorbic Acid). Pharmacol Pharm. 2013;4(1):84–92.
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Published
2023-05-10
How to Cite
Rahayu, A. U. (2023). Analysis of Protective Effects of Forest Honey (Apis dorsata) on Villi Height and Cryptus Depth of Duodenum in Wistar Male Rat Exposed to Aspirin. Jurnal Kedokteran, 12(1), 1308–1314. https://doi.org/10.29303/jku.v12i1.884
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