Pengaruh Pemberian Ekstrak Kulit Kacang Tanah (Arachis Hypogaea) Terhadap Kadar Trigliserida pada Tikus Putih (Rattus Norvegicus) yang diinduksi dengan Diet Tinggi Lemak
DOI:
https://doi.org/10.29303/jku.v6i2.1.223Keywords:
peanut shell extract (Arachis hypogaea), high fat diet, triglyceride, Rattus norvegicus.Abstract
Background: As a way to prevent cardiovascular disease, peanut shell has been develop to be an alternative way. The present bioactive compound, luteolin, acts as antioxidant and can also influence trygliceride metabolism. The purpose of this study is to find out the effect of the peanut shell extract (Arachis hypogaea) ontriglyceride level in rats (Rattus norvegicus) induced by high fat diet.
Method: Present research is an experimental research with post test control group only. The rats divided into 5 groups. They are negative control group induced by daily diet, positive control induced by simvastatin 0,9 mg/kgBB/daily, and three treatment groups induced by three different dosage of peanutshell extract (Arachis hypogaea). Three different dosage consist of 300mg/kgBB/daily (P1), 600 mg/kgBB/daily (P2), and 1200/kgBB/daily (P3) orally. Before the treatment start, we induced the rats with high fat diet 10mg/daily for 6 weeks to make hypertrygliceride state.
Result: Based on the experiment, the averages of triglyceride level among KN, KP, P1, P2, and P3 are 125,8 20,4 mg/dl, 80,2 44 mg/dl, 112,4 34,6 mg/dl, 159,4 46,8 mg/dl, dan 75,4 31,9 mg/dl.
Conclusion: Based on the result, it can be concluded that the oral induction of 300mg/kgBB/daily nutshell extract (Arachis hypogaea) has the same effect with simvastatin 0,19mg/kgBB/daily to lowering triglyceride level in mice with hypercholesterol state. While the oral induction of 1200mg/kgBB/daily nutshell extract (Arachis hypogaea) has the bigger effect than simvastatin 0,19mg/kgBB/daily on lowering triglyceride level in mice with hypercholesterol state.
References
2. Libby,P. 2011 The Vascular Biology of Atherosclerosis, dalam Mann., Zipes., Bonow., et al, vol.10 873-931.
3. Matsumoto, S., Gotoh, N., Hishinuma, S., 2014. The Role of Hypertriglyceridemia in the Development of Atherosclerosis and Endothelial Dysfunction. Nutrients
4. Price, S.A., Wilson, L.M., 2012. Patofisiologi Konsep Klinis Proses-Proses Penyakit Edisi 6. Jakarta: EGC
5. Kumar, S., Pandey, A., 2013. Review Article: Chemistry and Biological Activities of Flavonoid. The Scientific World Journal
6. Qiu, J., 2012. Screening natural antioxidants in peanut shell using DPPH-HPLC-DAD-TOF/MS methods. Food Chem 135(4):2366-71
7. Birari, R.B., Bhutani, K.K., 2007. Pancreatic Lipase Inhibitor From Natural Sources: Unexplored Potential. Drug Discovery Today Vol. 12
8. Moreno, D.A., Ilic, N., Poulev, A., et al., 2006. Effects Of Arachis Hypogaea Nutshell Extract On Lipid Metabolic And Obesity Parameters. Life Sciences 2797-2803.
9. Hadimi, L.I., Hizmi, S., Asdiani, H., Et Al., 2012. Efektifitas Ekstrak Etanol Kulit Kacang Tanah Terhadap Kadar Glukosa Darah Mencit Hiperglikemia Sebagai Langkah Awal Pencarian Obat Diabetes Mellitus Dari Limbah Bahan Pangan. Universitas Mataram (Skripsi).
10. Harsa IMS. 2014, Efek Pemberian Diet Tinggi Lemak Terhadap Profil Lemak Darah Tikus Putih (Rattus Norvegicus). Jurnal Ilmiah Kedokteran.
11. Dahlan, M.S., 2009. Teori Sederhana Prosedur Penelitian Uji Hipotesis, dalam Dahlan, M.S., Statistik Untuk Kedokteran dan Kesehatan, Salemba Medika, Jakarta
12. Akbar, A,T., 2013. Uji Aktivitas Ekstrak Etanolik Daun Kacang Tanah (Arachis Hypogaea L.) Terhadap Penurunan Kadar Trigliserida Serum Darah Tikus Putih Jantan Galur Wistar, Fakultas Farmasi, Universitas Setia Budi Surakarta (Skripsi)
13. Wardhani, R.R., 2012. Studi Terapi Ekstrak Air Daun Sukun (Artocarpus altilis) Terhadap Penurunan Kadar Trigliserida Dan Histopatologi Hepar Pada Tikus (Rattus norvegicus) Hiperkolesterolemia. Fakultas Kedokteran Hewan Universitas Brawijaya.
14. Han, L.K., et al. 2001. Anti-obesity effects in rodents of dietary teasaponin, a lipase inhibitor. International Journal of Obesity and Related Metabolic Disorders 25 (10), 1459–1464
15. Yoshikawa, M., Shimoda, H., Nishida, N., Takada, M., Matsuda, H., 2002.Salacia reticulata and its polyphenolic constituents with lipase inhibitory and lipolytic activities have mild antiobesity effects in rats. Journal of Nutrition 132 (7), 1819–1824.
16. Stancu, C., Sima, A., 2001. Statins: mechanism of action and effects. J Cell Mol Med. 2001 Oct-Dec;5(4):378-87.
17. Bolsoni, B.L., et al. 2015. Lipolysis and Lipases in White Adipose Tissue – An Update. Arch Endocrinol Metab. 2015;59/4.
