Polymorphism of Fat Mass and Obesity Associated (FTO) gene as a risk Factor for Type 2 Diabetes Mellitus with Metabolic Syndrome at DR Sardjito Hospital Yogyakarta
DOI:
https://doi.org/10.29303/jku.v3i2.741Kata Kunci:
Type 2 Diabetes Mellitus with metabolic syndrome, rs9939609 polymorphism, FTO geneAbstrak
Introduction . FTO gen is one of the new recently studied and still requires a lot of studies on the role of this gen on the incidence of metabolic syndrome. FTO rs 9939609 polymorphism in some populations have mentioned as the risk factors for obesity, type 2 diabetes mellitus and metabolic syndrome. Protein FTO role in demethylations of the genes in the hypothalamus that helps maintain energy intake and expenditure.
Methods This study used a case- control design with type 2 DM subjects with metabolic syndrome (n = 40) as cases and type 2 diabetes mellitus without metabolic syndrome (n = 40) as a control from DR.Sardjito Hospital Yogyakarta 2009-2010. FTO gen rs9939609 polymorphism was analyzed by PCR-RFLP. Chi square test and odds ratio test are used to examine FTO rs9939609 polymorphism and the relationship with the incidence of type 2 diabetes with metabolic syndrome and it’s components.
Results The frequency of TT genotype in subjects with type 2 DM patients with metabolic syndrome 2(5%), AT genotype 35 (87.5%) and AA genotype 3 (7.5%) (n = 40). In subjects with type 2 DM patients without metabolic syndrome TT genotype frequency 6 (15%), AT genotype 32 (80%) and AA genotype 2 (5%) (n = 40).OR are significant for sistolic hypertension (OR=0,039) and for HDL plasma level (OR=0,046) as the components of metabolic syndrome.
Conclusion The FTO gen rs9939609 polymorphism is the risk factor for hypertension and high HDL plasma level as the components of metabolic syndrome at DR Sardjito Hospital Yogyakarta.
Referensi
,Simposium Endokrinologi Klinik V, Bandung 18-20 Juni 2004.
2. Gerken T, Girard CA, Tung YL, Webby CJ, Saudek V, Hewitson KS, Yeo G, McDonough MA, Cunliffe S, McNeill L, Galvanovskis J, Rorsman, Robins, Prieur X, Coll A.P, Ma M, Jovanovic Z, Farooqi IS,Sedgwick B, Barroso I, Lindah I, Ponting CP, Ashcroft F.M, Stephen , Christopher J. 2007. The Obesity-Associated FTO Gene Encodes a 2-Oxoglutarate Dependent Nucleic Acid Demethylase. Science 318: 1469
3. Fredriksson R, Ha¨gglund M, Olszewski PK, Stephansson O, Jacobsson JA, Olszewska AM, Levine AS, Lindblom J, Schio¨th HB. 2008. The Obesity Gene, FTO, Is of Ancient Origin, Up-Regulated during Food Deprivation and Expressed in Neurons of Feeding-Related Nuclei of the Brain. Endocrinology 149(5):2062–2071
4. Andreasen C, Petersen KL, Mogensen MS, Torekov ST, Wegner, Andersen G, Nielsen AL, Albrechtsen A, Borch-Johnsen K, Rasmussen SS, Clausen J, Sandbæk A, Lauritzen A, Hansen L, Jørgensen K, Pedersen O and Hansen T. 2008. Low Physical Activity Accentuates the Effect of the FTO rs9939609 Polymorphism on Body Fat Accumulation, Diabetes 57:95–101
5. Speakman JR, Rance KA , Johnstone AM, 2008, Polymorphisms of the FTO Gene Are Associated With Variation in Energy Intake, but not Energy Expenditure, Obesity J 10:1038
6. Wahlen K, Sjo¨lin E, Hoffstedt J. 2008. The common rs9939609 gene variant of the fat mass and obesity-associated gene FTO is related to fat cell lipolysis , J. Lipid Res.49: 607–611.
7. Al-Attar SA, Pollex RL, Ban MR, Young TK, Bjerregaard T, Anand SS, Yusuf S, Zinman B, Harris SB, Hanley A, Connelly P, Huff M and Hegele RA. 2008. Association between the FTO rs9939609 polymorphism and the metabolic syndrome in a non-Caucasian multi-ethnic sample, Cardio Diabet. 7:5
8. Do R, Bailey, Desbiens K, Belisle A, Montpetit A, Bouchard C, Pérusse, Engert JC . 2008. Genetic Variants of FTO Influence Adiposity, Insulin Sensitivity, Leptin Levels, and Resting Metabolic Rate in the Quebec Family Study, Am Diabet J 57 : 1147-1150
9. PERKENI. 2006. Konsensus Pengelolaan dan Pencegahan Diabetes mellitus tipe 2 di indonesia 2006. Pengurus Besar Perkumpulan Endokrinologi Indonesia (PB.PERKENI)
10. Pulido LS, Navarro MA, 2007. The FTO (fat mass and obesity associated) gene codes for a novel member of the non-heme dioxygenase superfamily, BMC Biochem 8:23
11. Chang YC, Liu P, Lee W, Chang TJ, Jiang , Hung-Yuan, Kuo S, Lee KC and Chuang LM. 2008. Common Variation in the FTO Gene Confers Risk of Obesity and Modulates Body Mass Index in the Chinese Population, Am Diabet J 17:3
