Antibacterial Activity of Phenolic Compounds Fraction Robusta Coffee (Coffea canephora L.) Pulp Fraction
DOI:
https://doi.org/10.29303/jku.v12i3.952Keywords:
antibacterial, E.coli, phenolic, pulp, robusta, S.aureusAbstract
Coffee robusta pulp contain phenolic compunds that have biological effects especially as an antibacterial agent. The aim of this study was to determine the content of phenolic compounds and antibacterial activity from Staphylococcus aureus and Escherichia coli. The sampel was extracted and then fractionated with aquades and ethyl acetate. Qualitative analysis was analyzed by tube test and Thin Layer Chromatography (TLC). Antibacterial activity tested using the disc diffusion method with positive control (Chloramphenicol 1%), negative control (DMSO 10%), ethanol extract (concentration 20; 30; and 40%) and both fractions (concentration 2; 3; and 4%). The results of the analysis showed that the extract, water fraction, and ethyl acetate fraction contained phenolic compounds. The results of the antibacterial activity analyzed with SPSS using Kruskall-Wallis and Mann-Whitney test. Antibacterial test of the extract and ethyl acetate fraction were able to inhibited the growth of S. aureus, while only extract 40% and ethyl acetate fraction were inhibited the growth of E. coli. From this study, it can be concluded that ethanol extract and ethyl acetate fraction of coffee robusta pulp has potential as an antibacterial candidate.
References
Amalia, S., Wahdaningsih, S., & Untari, E. K. (2016). Uji aktivitas antibakteri fraksi n-heksan kulit buah naga merah (Hylocereus polyrhizus britton & rose) terhadap bakteri Staphylococcus aureus ATCC 25923. Jurnal Fitofarmaka Indonesia, 1(2), 61–64. https://doi.org/10.33096/jffi.v1i2.191
Anggita, D., Nuraisyah, S., & Wiriansya, E. P. (2022). Mekanisme kerja antibiotik. UMI Medical Journal, 7(1), 10–11. http://jurnal.fk.umi.ac.id/index.php/umimedicaljournal/article/view/149/121
Arpi, N., R, R., Widayat, H. P., & FOenna, R. F. (2018). Pemanfaatan limbah kulit buah kopi arabika (Coffea arabica l.) Menjadi minuman sari pulp kopi dengan penambahan sari jeruk nipis (Citrus aurantifolia) dan lemon (Citrus limon). Jurnal Teknologi Dan Industri Pertanian Indonesia, 10(2), 33–39. https://doi.org/https://doi.org/10.17969/jtipi.v10i2.12593
Febriyanto, F., Hanifa, N. I., & Muliasari, H. (2021). Penetapan kadar fenolik total ekstrak kulit buah kopi robusta (Coffea canephora L.) di Pulau Lombok. Lumbung Farmasi: Jurnal Ilmu Kefarmasian, 2(2), 89–95. http://journal.ummat.ac.id/index.php/farmasi/article/view/5036
Hafsah, H., Iriawati, I., & Syamsudin, T. S. (2020). Dataset of volatile compounds from flowers and secondary metabolites from the skin pulp, green beans, and peaberry green beans of robusta coffee. Data in Brief, 29, 105219. https://doi.org/10.1016/j.dib.2020.105219
Kusumastuti, M. Y., Meilani, D., & Tawarnate, S. (2021). Aktivitas Antibakteri Ekstrak, Fraksi Kloroform dan Fraksi n-Heksan Daun Kemangi terhadap Staphylococcus aureus dan Eschericia coli. Jurnal Indah Sain s Dan Klinis, 2(1), 17–22. https://doi.org/10.52622/jisk.v2i1.11
Mahbubah, Sri Peni Fitrianingsih, R. C. (2020). Uji aktivitas antidiare ekstrak etanol kulit buah kopi robusta (Coffea canephora pierre ex a. Froehner) terhadap mencit swiss webster jantan. Prosiding Farmasi, 6(1), 128–134. https://doi.org/10.29313/.v6i2.22573
Martauli, E. D. (2018). Analysis of coffee production in Indonesia. JASc (Journal of Agribusiness Sciences), 1(2), 112–120. https://doi.org/10.30596/jasc.v1i2.1962
Meilisnawaty, D., Suryanto, D., & Fauziah, I. (2015). Pemeriksaan Escherichia coli, Staphylococcus aureus dan Salmonella pada es jus jeruk examination. Jurnal Biologi Lingkungan, 2(1), 55–63. http://www.ojs.uma.ac.id/index.php/biolink/article/view/767
Mulyadi, M., Wuryanti, W., & Sarjono, P. R. (2017). Konsentrasi hambat minimum (KHM) kadar sampel alang-alang (Imperata cylindrica) dalam etanol melalui metode difusi cakram. Jurnal Kimia Sains Dan Aplikasi, 20(3), 130–135. https://doi.org/10.14710/jksa.20.3.130-135
Muzdalifa, D., & Jamal, S. (2019). Uji aktivitas antioksidan ekstrak fraksi kulit biji kopi robusta (Coffea canephora pierre ex a . Froehner) terhadap pereaksi dpph (1 , 1-difenil-2-pikrilhidrazil). Іndоnеѕіа Nаturаl Rеѕеаrсh Рhаrmасеutісаl Jоurnаl, 4(2), 41–50. https://core.ac.uk/download/pdf/268463407.pdf
Nabilla, I. I., & Indrayudha, P. (2019). Aktivitas sitotoksik ekstrak etanol, fraksi etanol, etil-asetat, dan heksana kulit jeruk purut (Citrus hystrix dc.) terhadap sel kanker payudara T47D. Pharmacon: Jurnal Farmasi Indonesia, 16(1), 11–17. https://doi.org/10.23917/pharmacon.v16i1.8173
Pratiwi, D. N., Utami, N., & Pratimasari, D. (2021). Identifikasi Senyawa Flavonoid dalam Ekstrak , Fraksi Polar , Semi Polar serta Non Polar Bunga Pepaya Jantan ( Carica papaya L .) Identification Flavonoids on Extract , Fraction Polar , Semi Polar and Non Polar of Male Papaya Flower ( Carica papaya L .). Jurnal Farmasi, 2(1), 1–7. https://ojs.stikesnas.ac.id/index.php/jf/article/view/152
Supeno, B., E, E., & Ernawati, N. M. L. (2018). Diversifikasi pemanfaatan limbah kulit buah kopi untuk produk yang bernilai ekonomis tinggi di kabupaten lombok utara. Fakultas Pertanian Universitas Mataram, 1, 23–25. http://www.prosiding-pkmcsr.org/index.php/pkmcsr/article/view/216
Suryanto, E., & Momuat, L. I. (2017). Isolasi dan Aktivitas Antioksidan Fraksi dari Ekstrak Tongkol Jagung. Agritech, 37(2), 139. https://doi.org/10.22146/agritech.27537
Suryanto, E., & Taroreh, M. R. I. (2020). Ultrasound-assisted extraction antioksidan serat pangan dari tongkol jagung (Zea mays L.). Chemistry Progress, 12(2). https://doi.org/10.35799/cp.12.2.2019.27932
Syamsul, E. S., Anugerah, O., & Supriningrum, R. (2020). Penetapan Rendamen Ekstrak daun Jambu Mawar Determination of Mawar Jambu Leaf Extract ( Syzygium. Jurnal Riset Kefarmasian Indonesia, 2(3), 147–157.
Wirasisya, D. G., Hajrin, W., & Handa, M. (2018). Aktivitas antibakteri ashitaba (Angelica keiskei) terhadap Streptococcus mutans. Jurnal Kedokteran, 7(2), 16. http://jku.unram.ac.id/article/view/180
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Jurnal Kedokteran
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
