Effect Of Variation Barbiturate (Sodium Phenobarbitone) Lethal Dose On Calliphoridae Larvae Length To Rat (Rattus Norvegicus) Carcasses Wistar Strain
DOI:
https://doi.org/10.29303/jku.v9i2.416Keywords:
PMI (post-mortem interval), Chrysomya sp. larval length, phenobarbital variation lethal dose, temperature, humidityAbstract
Background: Determining the time of death (post-mortem interval) is very important in forensic investigations. Blowflies growth is the main and most accurate indicators time of death to estimation PMI. The growth of flies can be affected by various factors including climate, geography, and medicines contained in the body. The aim of this study was to examine the effect of giving variations barbiturate (sodium phenobarbitone) lethal dose to the length of Calliphoridae larvae to predicting PMI.
Methods: This study is an experimental study with post-test only control group design, the data were analyzed using multivariate linear regression analysis test. This study consisted of 1 control group (neck dislocation) and 3 control groups of phenobarbital (750 mg/kgBW, 900 mg/kgBW and 1050 mg/kgBW). All group are put into a cage containing Chrysomya sp. The measurements of larva length are carried out every 12 hours by taking the largest larvae size in each rat in the study group.
Result: The results shown that phenobarbital was the most influential factor in the larval length of Chrysomya sp. (p <0.05, R = -0.64) compared to temperature and humidity. Mann Whitney test results (p <0.05) shown the significant difference in Chrysomya sp. larvae length in each study group.
Conclusions: Variation lethal dosage of barbiturates (sodium phenobarbital) affects the growth of Chrysomya sp larvae length.
References
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4. Verma K & Paul R, 2013. Assessment of Post Mortem Interval (PMI) from Forensic Entomotoxicological Studies of Larvae and Filies. Entomol Ornithol Herpetol 2.doi: 10.4172/2161-0983.1000104 Available at: https://www.longdom.org/open-access/assessment-of-post-mortem-interval-pmi-from-forensic-entomotoxicological-studies-of-larvae-and-flies-2161-0983.1000104.pdf [Diakses pada 24 Februari 2019]
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6. Gautam B., 2015. Forensic Medicine and Toxicology. 3rd ed. Philadelphia: The Heatlh Sciences Publisher. pp. 126-156 Availabe at: https://b-ok.cc/book/2604887/c80bab [Diakses pada 19 Februari 2019]
7. Dahlem, G.A., 2014. The Science of Forensic Entomology. 1st ed. Wiley-Blackwell. pp.69-91,210 Available at: https://b-ok.cc/book/2335319/184c57 [Diakses paa 19 Februari 2019]
8. Payne J.J., 2011. Simpson’s Forensic Medicine. 13th ed. pp. 50-53 Available at: https://scm2016.files.wordpress.com/2014/05/simpson-forensic-medicine.pdf [Diakses pada 19 Februari 2019]
9. Velez Mc&Wolff M., 2008. Rearing five species of Diptera (Calliphoridae) of forensic importance in Colombia in semicontrolled field conditions, Papeis Avulsos de Zoologia, vol 48, no.6, pp.41-7 [pdf] Available at: http://www.scielo.br/pdf/paz/v48n6/a01v48n6.pdf [Diakses pada 14 April 2019]
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11. Rezende F, Alonso Ma, Souza CM, Thyssen PJ, LInhares AX., 2014. Developmental rates of immatures of three Chrysomya species (Diptera: Calliphoridae) under the effect of methylphenidate hydrochloride, phenobarbital, and methylphenidate hydrochloride associated with Phenobarbital [pdf] Available at: https://www.ncbi.nlm.nih.gov/pubmed/24633905 [Diakses pada 9 Mei 2019]
12. Almulhim AM & Menezes RG, 2019.Postmortem Changes. StatPearls Publishing [online] Available at: https://www.ncbi.nlm.nih.gov/books/NBK539741/ [Diakses pada 18 November 2019]
13. Wells J D & Lamotte L, 2001. Estimaing he Postmortem Interval [pdf] Available at: https://www.researchgate.net/publication/273379119_Estimating_the_Postmortem_Interval/link/54ff509f0cf2eaf210b90992/download [Diakses pada 19 November 2019]
14. Zhang Y, Wang Y, Yang L, Tao L & Wang J, 2018. Development of Chrysomya megacephala at constant temperatures within its colony range in Yangtze River Delta region of China [pdf] Available at: https://www.ncbi.nlm.nih.gov/pubmed/30483654 [Diakses pada 22 November 2019]
15. Gosselin et al., 2011. Entomotoxicology, experimental set-up and interpretation for forensic toxicologists. Forensic Science International.Elvesier Ireland Ltd.pp.2-4 [pdf] Available at: https://doi.org/10.1016/j.forsciint.2010.10.013 [Diakses pada 8 Mei 2019]
16. Lewis CB, Adam N., 2019. Phenobarbital.In: StatPearls. Teasure Island (FL): StatPearls Publishing [Online] Available at: https://www.ncbi.nlm.nih.gov/books/NBK532277/ [Diakses pada 22 April 2019]
17. National Institutes of Health, 2019. Phenobarbital [Online] Available at: https://pubchem.ncbi.nlm.nih.gov/compound/phenobarbital [Diakses pada 22 April 2019]
18. Gilbert SF, 2000. Developmental Biology 6th edition. Sinauer Associaties [pdf] Available at: https://www.ncbi.nlm.nih.gov/books/NBK9983/ [Diakses pada 4 Desember 2019]
19. World Health Organization, 2001. IARCMonographs on the Evaluation of Carcinogenic Risks to Humans.Vol 79 [pdf] Available at: https://monographs.iarc.fr/wp-content/uploads/2018/06/mono79.pdf [Diakses pada 4 Desember 2019]
20. Cheng Zhe, Liu Fan, Li Xiu, Dai Mengya, Wu Jianjuan, Guo Xinrui, Tan Huimin, Heng Zhijie, Lu Ying, Chai Xiaoli Wang Yanhai., 2017. EGF-mediated EGFR/ERK signaling pathway promotes germinative cell proliferation in Echinococcus multilocularisthat contributes to larval growth and development [pdf] Available at: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005418 [Diakses pada 5 Desember 2019]
21. Algalil F, Zambare S & Mail L, 2016.Effect of Seasonal Temperature Variations on the Life Cycle Duration of Forensically Important Calliphorid Fly, Chrysomya saffranea (Bigot,1877) [pdf] Available at: https://www.omicsonline.org/open-access/effect-of-seasonal-temperature-variations-on-the-life-cycle-duration-offorensically-important-calliphorid-fly-chrysomya-saffranea-2157-7145-1000364.php?aid=85097 [Diakses pada 23 November 2019]
22. Ralebitso K T., 2018. Forensic Ecogenomics: Tha Application of Microbial Ecology Anayses in Forensic Contexts.London Wall. Elsevier.pp.116 [Online] Available at: https://books.google.co.id/books?id=dyk0DwAAQBAJ&pg=PA243&lpg=PA243&dq=forensic+ecogenomics:+The+Application+of+Microbial+Ecology+Analyses+in&source=bl&ots=75Ydy8sJla&sig=ACfU3U3ibKQk9SSkPlH7DnHhrLMhAcE-mg&hl=id&sa=X&ved=2ahUKEwiejdLesrziAhUmgK0KHbqmBmA4ChDoATAGegQICRAB#v=onepage&q=forensic%20ecogenomics%3A%20The%20Application%20of%20Microbial%20Ecology%20Analyses%20in&f=false [Diakses pada 9 Mei 2019]
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Published
2020-08-12
How to Cite
Dwi Astuti Wulandari, Syamsun, A. ., & Triani, E. . (2020). Effect Of Variation Barbiturate (Sodium Phenobarbitone) Lethal Dose On Calliphoridae Larvae Length To Rat (Rattus Norvegicus) Carcasses Wistar Strain. Jurnal Kedokteran, 9(2), 111–123. https://doi.org/10.29303/jku.v9i2.416
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