PENGARUH EKSPRESI RESEPTOR INSULIN PANKREAS TERHADAP EKSPRESI GLUT4 NEURON HIPOKAMPUS PADA TIKUS MODEL DIABETES

Authors

  • Aliza Raudatin Sahly PSPD Fakultas Kedokteran Universitas Mataram
  • Ilsa Hunaifi
  • Rizka Vidya Lestari
  • Indah Sapta Wardani
  • Novrita Padauleng
  • Nurhidayati
  • Herpan Syafii Harahap

DOI:

https://doi.org/10.29303/jku.v9i4.441

Keywords:

GLUT$, reseptor insulin, diabetes

Abstract

Background: Diabetes Melitus (DM) is a degenerative diseases characterized by hyperglycemia condition. Basic Health Research data for 2018, shows an increase in prevalence of DM in Indonesia over last 5 years with 6,9% in 2013 to 8,5% in 2018. Pancreatic insulin receptor expression can be a marker for assessing presence of ? cells, production and secretion of insulin. Insulin could cause GLUT4 to be translocated to cell membrane of hippocampal neurons. In DM conditions, there may be changes in expression of pancreatic insulin receptors accompanied by changes in expression of GLUT4 hippocampal neurons.

 

Methods: This experimental research used 16 white rats (Rattus Novergicus) which divided randomly into 4 groups, namely 2 control grups (A0 and A1) and 2 treatment groups (B0 and B1). Treatment group were induced by diabetes with a single dose of nicotinamide 110 mg/kgBB and streptozotocin 70 mg/kgBB intraperitoneally. The research data were obtained from immunohistochemical analysis of pancreatic and hippocampal tissue.

 

Result: The mean expression of pancreatic insulin receptor in diabetic rat group was higher but not significant on the 14th day observation compared to the 0th day (p>0,05), while the mean GLUT4 expression of hippocampal neurons was significantly higher on 14th day of observation compared to the 0th day (p<0,05). The result of correlation test did not show a significant results in each group both at the 0th and 14th day observation (p>0,05).

 

Conclusion: There was no significant effect of pancreatic insulin receptor expression on hippocampal neuron GLUT4 expression in diabetic model rats.

References

1. Triplitt, C. L. Examining the mechanisms of glucose regulation. The American Journal of Managed Care, 18(1 Suppl). 2012;S4-10.
2. PERKENI. Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 di Indonesia, PERKENI, Jakarta. 2019.
3. International Diabetes Federation. IDF Diabetes Atlas Ninth Edition 2019. Dunia : IDF. 2019.
4. Riset Kesehatan Dasar (Riskesdas). Badan Penelitian dan Pengembangan Kesehatan Kementerian RI tahun 2018. 2018.
5. Liu, C., Hu, J., Tsai, C, W., Yue, M., Melrose, H. L., Kanekiyo, T., & Bu, G. Neuronal LRP1 Regulates Glucose Metabolism and Insulin Signaling in the Brain. Journal of Neuroscience. 2015;35(14), 5851–5859.
6. Fu, Z., R. Gilbert, E., & Liu, D. Regulation of Insulin Synthesis and Secretion and Pancreatic Beta-Cell Dysfunction in Diabetes. Current Diabetes Reviews. 2012;9(1), 25–53.
7. Anita, M., Deborah, J., Umut, O., Rohit, N., Jing, Y., Sunmin, P., Morris, F. Upregulation of insulin receptor substrate-2 in pancreatic ? cells prevents diabetes. Journal of Clinical Investigation. 2003;112(10).
8. Rhodes, CJ, Moris, W, Leahy, J & Khan S. Direct Autocrine Action of Insulin on Beta Cells : Does It Make Physiological Sense?. Departement of Endocrinology. 2018;Vol 62.
9. Folli, F., Okada, T., Perego, C., Gunton, J., Liew, C. W., Akiyama, MKulkarni, R. N. Altered insulin receptor signalling and ?-cell cycle dynamics in type 2 diabetes mellitus. PLoS ONE. 2011;6(11).
10. Kulkarni, R., Kulkarni, R., Brüning, J., Brüning, J., Winnay, J., Winnay, J., Ma. in Pancreatic Cells Creates an Insulin Secretory Defect Similar to that in Type 2 Diabetes. Cell-Cambridge Ma. 1999;96, 329–339.
11. Haribal, M. L., Perego, L., Lovari, S., Andreozzi, F. Chronic hyperglycemia impairs insulin secretion by affecting insulin receptor expression, splicing, and signaling in RIN beta cell line and human islets of Langerhans. The FASEB Journa : Official Publication of the Federation of American Societies for Experimental Biology l. 2003; 17(10), 1340–1342.
12. Ashrafi, G., Wu, Z., Farrell, R. J., & Ryan, T. A. GLUT4 Mobilization Supports Energetic Demands of Active Synapses. Neuron. 2017.
13. Reno, C. M., Puente, E. C., Sheng, Z., Daphna-Iken, D., Bree, A. J., Routh, V. H., Fisher, S. J. Brain GLUT4 knockout mice have impaired glucose tolerance, decreased insulin sensitivity, and impaired hypoglycemic counterregulation. Diabetes. 2017;66(3), 587–597.
14. Sompuram, S.R., Vani, K., Schaedle, A.K., Balasubramanian,A., Bogen, S.A. Quantitative Assessment of Immunohistochemistry Laboratory Performance by Measuring Analytic Response Curves and Limits of Detection. Arch Pathol Lab Med. 2018;Vol.142, pp.851-862.
15. Jiang, W. J., Peng, Y. C., & Yang, K. M. Cellular signaling pathways regulating ?-cell proliferation as a promising therapeutic target in the treatment of diabetes (Review). Experimental and Therapeutic Medicine. 2018;16(4), 3275–3285.
16. Ghasemi, A., Khalifi, S., & Jedi, S. Streptozotocin-nicotinamide-induced rat model of type 2 diabetes (review). Acta Physiologica Hungarica. 2014; 101(4), 408–420.
17. Nurdiana, S., Goh, YM., and Mahdi, E. Changes in Pancreatic Histology, Insulin Secretion and Oxidative Status in Diabetec Rats Following Treatment with Fiscus Deltoidea and Ventin. BMC Compleentary and Alternative Medicine. 2017.
18. Zhong, F., Jiang,Y. Endogenous Pancreatic B cell Regeneration: Potential Strategy for the Recovery of B Cell Deficiency in Diabetes. 2019.
19. Leary, J.P., and McNay, E.C. GLUT4 : A central player in hippocampal memory and brain insulin resistance. Experimental Neurology. 2019.
20. Harahap, H.S., Padauleng, N., Nurhidayati, dan Ekawanti, A. Ekspresi GLUT4 pada Neuron Hipokampus Rattus novergicus Diabetik yang Diinjeksi Streptozotocin dan Nicotinamide. Jurnal Kedokteran Brawijaya. 2019;Vol. 30 (3).
21. Dastbarhagh, H., Kargarfard, M., Abedi, H., Bambaechi, E., and Nazarali P. Effects of Food Restriction and /or Aerobic Exercise On The GLUT4 In Type 2 Diabetic Model Rats. Departement Of Exercise Physiology University od Isfahan. 2019.

Downloads

Published

2021-01-11

How to Cite

Sahly, A. R., Hunaifi, I., Lestari, R. V., Wardani, I. S., Padauleng, N., Nurhidayati, & Harahap, H. S. (2021). PENGARUH EKSPRESI RESEPTOR INSULIN PANKREAS TERHADAP EKSPRESI GLUT4 NEURON HIPOKAMPUS PADA TIKUS MODEL DIABETES. Jurnal Kedokteran, 9(4), 307–316. https://doi.org/10.29303/jku.v9i4.441

Issue

Section

Research

Most read articles by the same author(s)

<< < 1 2 3 4 > >>